Steep Hill Halent: Cannabis Testing Laboratory | California, Colorado, Washington | Medical marijuana analytical, research lab | Quality assurance, safety screening | Cannabinoid, terpenoid, THC, CBD, residual solvent RSA, pesticide, mold
Many who have heard of the cannabinoid Cannabinol (CBN) have a negative impression of the compound, as it is typically believed to be a degraded compound that appears in old, poorly stored cannabis. However, this bleak perspective is not entirely accurate.
Unlike most cannabinoids, CBN rarely results from the decarboxylation of its acid form (CBNA). Most of the CBN found in cannabis is due to the degradation of THC, wherein the THC has oxidized to CBN.
CBN is either non-psychoactive or very mildly psychoactive. It reduces intraocular pressure in the eye (similar to D9THC), so it can be used in the treatment of Glaucoma without the psychoactive effects of THC. CBN is synergistic with THC for treatment purposes. CBN also fights free radicals in the bloodstream, and it performs similarly to THC in pain reduction treatments.
Of all the cannabinoids, CBN appears to be the most sedative. Not only is it sedative, it takes very little to do the job. The consumption of 2.5mg to 5mg of CBN has the same level of sedation as a mild pharmaceutical sedative, with a relaxed body sensation similar to 5mg to 10mg of diazepam. CBN is synergistic with both CBD and D9THC for inducement of sleeping, and when mixed in the correct ratios, CBN becomes an effective sleep aid of 5-6 hours duration.
As we do more research focused on CBN, we will no doubt discover more fascinating properties of this often misunderstood cannabinoid. In the meantime, CBN appears to be a beneficial alternative to THC for treatment, and patients that require low (or no) adverse psychoactive effects.
Download full PDF report — Tangie Cookies Oil
Read more — Indica or Sativa: Here’s How to Know
Lemberger, L., and H. Rowe. “Clinical pharmacology of nabilone, a cannabinol derivative.” Clinical pharmacology and therapeutics 18.06 (1975): 720-726.
Boissier, J. R. “Clinical pharmacological study and eeg changes of delta-9 tetrahydro-cannabinol effects in human volunteers.” Biochemical Pharmacology23 (1974): 751-755.
Microbiological Screening at Steep Hill Halent
Molds (fungus) are living organisms that are often involved with the decomposition of organic matter. During the lifespan of a mold, it produces chemicals, by-products, and metabolites (like the alcohol produced by yeast during beer fermentation), including mycotoxins. Mycotoxins are so-named because they have been shown to be highly toxic to humans, animals, and plants. Along with acute toxicity (an immediate illness), continuous exposure to a specific mycotoxin often results in an allergic reaction resulting from buildup over time, that has the potential to be lethal to some patients. Once a patient becomes sick with a mycotoxin-induced type of allergy, the patient can remain allergic, becoming progressively sicker with each new exposure. Testing for mycotoxins — and removing them from cannabis flowers — is critical.
The most commonly seen mold is mildew, which luckily is also the least harmful. For most cannabis consumers, mildew has nothing more than a very unpleasant taste, but to those with lung impairments, compromised immune systems, or allergies to molds, mildew can become a serious problem. Making matters more serious, in general terms, very little research has been done to date to measure the effects of smoking or inhaling mold or mold spores into the lungs, so we have very little understanding of how serious these issue are.
Aspergillus is a very toxic mold sometimes seen in cannabis. Aspergillus can be black, green, brown, white, yellow or blue in color. Some strains of Aspergillus contain a highly toxic mycotoxin called Ochratoxin. Ochratoxin is carcinogenic, destroys the kidneys, and causes neuropathic pain throughout the body. Routine laboratory testing for Aspergillus and Ochratoxin is done on coffee beans, red wine, cereals and dried fruits because of these concerns.
Another commonly-appearing Aspergillus strain is a mycotoxin called Aflatoxin, which is among the most carcinogenic chemicals known. Aflatoxin has been found in the breast milk of mammals eating contaminated feed, peanut butter, cooking oils such as olive oil, and in patients using contaminated cosmetics. Liquid chromatography (LC) testing has shown at least faint traces of Aflatoxin in at least 50% of food samples tested.
There are a variety of other molds and mycotoxins of concern. Under normal circumstances, the U.S. government, (through the F.D.A.) does an excellent job screening out these toxins from our food and drug supplies through mandatory testing. Unfortunately, since cannabis is still listed as a Schedule 1 substance, no mycotoxin testing is mandated by our government to protect us from these illnesses. Also, as mentioned previously, almost no testing has been specifically conducted to test the impact of inhalation of these molds and mycotoxins.
Cannabis patients and consumers need to be their own best advocate, and get in the habit of asking if the cannabis products they use are tested for mycotoxins, and if not, advise their supplier to test for mycotoxins.
Steep Hill Halent has finished analyzing kief from Doug’s Varin strain. Below are 2 lab reports; the first is unheated, and the second decarboxylated.
In the unheated sample, the THCA:THCVA ratio is a whopping 5:4, with 19% THCA and 15% THCVA. This means that each gram of this kief contains 190mg of THCA and 150mg of THCVA. This ratio is the lowest we have seen in this strain as other samples previously tested had slightly more THCVA than THCA.
Also note the 1.5% Myrcene. This large amount of Myrcene allows THC and THCV to enter the brain more quickly and in larger concentrations. Normally, with that level of Myrcene content, one would expect the kief to have a strong couch lock effect, but as it turns out, the extremely energetic effects of THCV negate the couch lock effect entirely.
When consumed, Doug’s Varin is exceptionally robust in its energetic properties, and also highly appetite suppressive. It is also very clear-headed, similar to Super Silver Haze, except Doug’s Varin delivers a more energetic reaction over a shorter duration as compared to Super Silver Haze.
The mother plant of this exceedingly rare strain has now been stabilized by three different expert horticulturists, who ultimately plan to release this strain for use in treatment of P.T.S.D., Parkinson’s, and other ailments for which it is exceptionally suited.
Download full PDF report — Doug’s Varin Kief
Download full PDF report — Doug’s Varin Kief [Decarboxylated]
Read more — THCV: The Sports Car of Cannabinoids
Rev. Dr. Kymron deCesare Chief Research Officer Steep Hill Halent
NextMarket Podcast with Michael Wolf
Guest: David Lampach, Steep Hill Halent.
“While Mike doesn’t use pot, he’s been fascinated to watch the unfolding of a new legal market for it in his home state of Washington after voters approved the legal sale of pot at retail in 2012.
So when he was pitched a chat with Steep Hill’s CEO Dave Lampach, he said why not? After all, there’s a good chance legal market will be a multibillion market in the coming years, and there’s a gold rush in terms of new VC and investor money pouring into the pot-tech space.”
Purple Phenotypes refer to plants that express ‘purple’ chlorophyll. Some plants developed this as a survival strategy in low-light environments.
For the most part, purple phenotypes may be divided into two basic categories. In the first category, the plant automatically starts producing the purple color at a specific time in the plant’s life cycle. In other words, it is a pre-programed event that the plant consistently activates; many growers are familiar with this.
The second category of purple phenotypes is triggered by temperature. When nighttime temperatures drop to (or below) 55 °F for at least 3-4 days, it triggers the production of the purple chlorophyll. This is why, especially for sativas with lengthy flowering times grown outdoors, some cannabis strains start changing color in late October and early November. These winter months are colder with shorter days. Once the purple chlorophyll production process has started, if temperatures rise, the purple chlorophyll remains. Some of Steep Hill Halent’s clients have learned that chilling indoor grows with an air conditioner while their plants are resting during their dark time works well to express this purple color characteristic.
Purple phenotypes basically come in 3 different colors: purple, red-brown, and bluish purple (indigo). Distinguishing the difference between these colors is useful in typing phenotypes.
Download full PDF report — Purple Urkle
Read more — Indica or Sativa: Here’s How to Know